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| Manufacturer |
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| type |
Nano Liquid Chromatography(nLC) |
| Name |
EASY - nLC II  |
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| Effortless, split-free nano-LC |
| for top performance in LC-MS |
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| Proven, split-free, nano-flow performance |
| Excellent high resolution results – every time |
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| - Accurate label-free quantification ensured by minimal RSD values for retention times |
| - Stable, pulsation-free gradients from 100 nL/min |
| - Advanced Flow ControlTM with two flow sensors prior to mixing |
| - Split-free gradient mixing – no flow splitting |
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| Fast separations with typical peak widths: 3.5 sec (FWHM) |
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| Low retention time RSD values for complex mixtures |
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| For SHRP QC testing, sample throughout is of paramount importance. Therefore, every feature of the DSRII design has been implemented with the goal of minimizing the time required to make each measurement according to AASHTO TP5. |
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“LC-MS experts and biologists alike require trouble-free operation and ease of use along with top performance. Experience has shown that the EASY-nLC system
readily meets those high demands.” |
| Professor Matthias Mann, Max Planck Institute for Biochemistry, Martinsried, Germany |
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| Efficient 2D LC-MS protein identification |
| Identify more proteins compared to conventional approaches |
| Identifies ~4X more proteins than 1D and ~20% more proteins than conventional 2D-LC |
| Venn diagram of identified number of proteins from analyses using the EASY-nLC system 2D configuration, conventional 2D (quaternary split-flow solvent delivery) or a 1D configuration. Sample: tryptic digest of a human multiple myeloma cell line protein extract. |
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| Minimal mixing and dilution of salt solutions during stepwise elution from SCX column |
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| Spray current profiles measured at MS inlet during salt injections using Thermo Fisher LTQ OrbiTrap XL. |
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2D configuration
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| Excellent sensivity and reproducibility |
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| Superior sample handling - precise pick-up, low carry-over of integrated autosampler |
| - Efficient Peltier-controlled sample cooling |
| - Injection volume selectable in 0.01 μl steps |
| - Injection needle washed outside and inside |
| - Custom wash with 2 extra solvent bottles |
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| Unrivalled ease of use and productivity |
| Installed and running within hours |
| - Quality assured - system pre-configured and QC-tested with MS |
| - Simple to interface and synchronize with MS |
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| Efficient operation |
| - Flow sensors immediately before high-pressure mixing ensure accurate delivery of each mobile phase |
| - Uses intelligent flow control to run according to user-defined pressure |
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rapid build-up of pressure and flow |
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active during equilibration and sample loading |
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avoids interruptions caused by exceeding maximum column pressure limits |
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analysis continues despite increasing column back pressure |
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| “We are extremely happy with the reliability and reproducibility of the EASY-nLC system.” |
| Kelly Hogue, Lab Manager, Mass Spectrometry (WB) Group, Institute of Molecular and Cell Biology, Singapore |
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| Easy maintenance, access and service |
| “...It’s so simple to use, I even installed the second one myself. And their service has been excellent.” |
| John M. Asara, Mass Spectrometry Core, Beth Israel Deaconess Medical Center, Boston, MA. |
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| Built-in PC & software |
| - automated, built-in maintenance steps |
| - remote access via internet for system interrogation |
| - event log for troubleshooting |
| - automated leak test indicates actual position of leak |
| - system back pressure test quickly detects flow-line blockage |
| Modular design for easy component exchange |
| Pulsation-free pumps minimize need for replacement of seals, valves and fittings |
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| Easily switch between 1 or 2 column configuration |
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| Sample Preparation and Purification |
| Fast, fail-safe purification of peptide and protein samples |
| Highest quality results are critically dependent on optimal sample preparation. Ready-to-use microcolumns, StageTips, are used for desalting, pre-concentration, and separation of samples for MALDI, LC-MS and nano ES-MS. |
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| Ensure cost-efficiency |
| - save time and eliminate errors associated with alternative solutions |
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| Choose from C8, C18 or SCX (strong cation exchange) chromatographic media |
| - preselected to ensure minimal unspecific and irreversible binding |
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| Follow a simple, effective generic protocol |
| - developed to ensure efficient binding in a single pass |
| - load, wash and elute at >300 μL/min using low back pressure |
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| Achieve excellent sensitivity in the low femtomole to high attomole range |
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| Ensure high reproducibility |
| - no tip variation, reliable protocols and superb ease of use |
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| Increase throughput |
| - ready-made, disposable tips are ideal for lab robots and multi-channel pipettes |
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| Chromatographic excellence at nanoscale |
Using top quality media and components, and produced with a focus on design simplicity and strict quality control,
EASY-ColumnTM capillary columns ensure top chromatographic performance at nanoscale. These columns represent the first of a new product range from Proxeon. |
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| Compatible with any nanoscale HPLC system |
| Optimized for online LC-MS |
| Quality control on every column |
| Simple, flexible design |
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| More information from proxeon : http://www.proxeon.com/home/index.html |
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| For more information contact : info@scispec.co.th |
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